Drimmer-Fischler Family Stem Cell Core Laboratory for Regenerative Medicine
Research & Initiatives
Implementation of existing protocols and the development of new protocols for the effective differentiation of hiPSCs to scalable and reproducible cell types useful for basic and translational research studies.
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We apply 2-dimensional and 3-dimensional cell culture platforms that can be used for complex, multi-cell type cultures as well as in-vitro organ systems.
Establishing a human relevant Brain-On-a-Chip platform for elucidating pathways and cellular dysfunction in Parkinson’s disease. Data obtained from
research groups headed by Prof. Uri Ashery and Dr. Ben Maoz.
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Identification of human iPSC-derived DA neurons TH, EN1 and TUJ1 immunostaining showing human iPSCs-derived DA neurons after 36 days of differentiation. Scale bar, 100μm.
Calcium imaging of human iPSC-derived DA neurons day 36 of differentiation (A) Human iPSCs derived DA neurons loaded with Fluo-4-AM. Scale bar, 20μm. (B) Recording of intracellular calcium level during spontaneous activity (blue trace) compared to changes in intracellular calcium in response to KCl (10 mM; orange trace) Scale bar, 20 μm.